Composite
"The Blue

Part:BBa_K498000:Design

Designed by: IvyTech_South-Bend Team   Group: iGEM10_IvyTech-South_Bend   (2010-10-26)
Revision as of 03:56, 28 October 2010 by Geotwaddle (Talk | contribs) (→‎Source)

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T9002 - LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1956
    Illegal BamHI site found at 1965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 1732
    Illegal BsaI.rc site found at 2902


Design Notes

The IvyTech_South-Bend Team created this hybrid part to grant the AHL - detector device T9002 and enzymatic/cholorimetric read out to (hopefully) to enable the design of a simplified (in the field) detection system (no fluorescence excitation required). The inclusion of the beta-gal mediated conversion of the IPTG substrate to a yellow color should serve this purpose.

Source

Registry of Standard Parts


BBa_T9002 (GFP Producer Controlled by 3OC6HSL Receiver Device) and BBa_I73200 (Lac Z)are Registry Parts that were created by Anna Labno (Haseloff lab) and Zhan Jian Group: iGEM07_USTC respectively.