DNA

Part:BBa_K371052

Designed by: Ruijun Zhu   Group: iGEM10_USTC   (2010-10-27)
Revision as of 03:37, 28 October 2010 by Registry (Talk | contribs)

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RFC 53 easy expression biobrick

This part locate in BBa_K371051, which is very like pSB1C3 with only a single site nonsense mutation between terminator 0 and chloramphenicol resistance gene. The function of this gene is to convert the any RFC53 part to RFC 10 part without leaving any trace. User will find it is very convenient to make fusion protein with this noval system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 238
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 244
    Illegal SapI.rc site found at 231


[edit]
Categories
Parameters
n/aRFC 53 easy expression biobrick