Measurement

Part:BBa_K313020

Designed by: Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 03:37, 28 October 2010 by Yuka (Talk | contribs)

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terminator leakiness assay device

This is a device that aims to measure the leakiness of terminator BBa_B0014

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.

The difference between this part and BBa K313019 is the number of the terminator.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 637
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 323
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None