Measurement
Part:BBa_K313019
Designed by: Ryosuke Kamei, Mami Nakatake, Ryo Kariyazono Group: iGEM10_UT-Tokyo (2010-10-22)
terminator leakiness assay device
This is a device that aims to measure the leakiness of terminator BBa_B0014
If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.
With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.
Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 534
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 220
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |