Coding

Part:BBa_K313002

Designed by: Kohaku So, Takashi Hiroi, Megumi Iseki, Mami Nakatake, Ryosuke Kamei, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-14)
Revision as of 03:10, 28 October 2010 by Ryo.T (Talk | contribs)

flpe DNA recombinase

Flpe is a site-specific DNA recombinase that recognizes FRT sequence. Flpe was derived from yeast site-specific DNA recombinase Flp. (Buchholz, et al.,Improved properties of FLP recombinase evolved by cycling mutagenesis. Nature. Biotechnology, 16:657-662 ( 1998 ))

We successfully removed the internal EcoRI and SpeI sites using site-directed mutagenesis.

Please see [http://2010.igem.org/Team:UT-Tokyo/Sudoku_assay_LeakSw Terminator leak] assay page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 504
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/recombination/flp
Parameters
None