Project

Part:BBa_K404204

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 02:21, 28 October 2010 by Kerstin (Talk | contribs)

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ViralBrick-453-Z34C

ViralBrick-453-Z34C)
BioBrick Nr. BBa_K404204
RFC standard RFC 25
Requirement pSB1C3
Source
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]















The Z34C antibody binding motif, ready for insertion into the 453 loop

Freiburg10 ViralBrick-logo-453-Z34C.png


Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of [http://www.ncbi.nlm.nih.gov/protein/153106 Staphylococcal Protein A]. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Freiburg10 ViralBrick motif Z34C.png


Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 453 [http://www.neb.com/nebecomm/products/productR3132.asp SspI-HF] Freiburg10 recognition site SspI-HF.Gif 25;100;0;100 37°C
downstream 453 [http://www.neb.com/nebecomm/products/productR3138.asp SalI-HF] Freiburg10 recognition site SalI-HF.Gif 10;100;100;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/capsid_coding
Parameters
None