Part:BBa_K318513
gadA/RBS + RFP + TT
uc
Usage and Biology
Low pH/Stationary Phase Induction
Download information (.zip) here
Below are two figures of sample data:
Figure 4. Lines represent OD600 and are associated with the right axis. Dots indicate RFU/OD600 and associated with the left axis. In this trial, maximum induction occurred at an OD600 of approximately 6 (right axis, associated with line) and yielded about 10000 RFU/OD. Note that immediately before maximum OD600 was reached, RFU/OD increased about 5-fold. Controls included for this trial were a lacI promoter + RBS + mRFP1 + TT construct in the same plasmid (pSB1A2) and a WT control of MG1655 cells. These controls and results indicate that (1) mRFP1 expression by gadAp at high OD600 is a function of the gadA promoter, not of promoters at large (lacI promoter did not express) or of the cells themselves (MG1655 did not fluoresce).
Figure 5.This trial was run parallel to the pH 7 trial in Figure 4. It indicates that cells grown in LB media at pH 5.5 do not exhibit a drastically different promotion behavior from their gadA promoters than those grown in neutral media.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 825
Illegal AgeI site found at 937 - 1000COMPATIBLE WITH RFC[1000]
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