Part:BBa_K318500
lacI pL + RBS + RcsA + TT
Our goals is to have each cell produce enough colonic acid to protect itself from the harsh acidic conditions of the stomach. We placed RcsA, RcsB, and a combination of the two under a IPTG inducible promoter to test both quantity of colonic acid produced and cell survivability. You can see our data by downloading the associated .zip file for any of these three devices: BBa_K318500(RcsA), BBa_K318501(RcsB), BBa_K318502(RcsA+RcsB).
Usage and Biology
RcsA is a transcription factor that is know to be a positive regulator of capsular polysaccharide synthesis. Along with transcription factor RcsB, these two transcription factors form a heterodimer that is know to activate around 19 genes related to colonic acid synthesis. See more on http://biocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10820 Ecocyc-RcsA
Characterization
Download Zip file media:BBa_K318500_pSB1C3.zip.
Cell Survivability in Acidic Conditions
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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