Part:BBa_K404319

Cyan fluorescent protein (CFP) in RFC25 standard

Cyan fluorescent protein
BioBrick Nr.	BBa_K404319
RFC standard	RFC 25
Requirement	pSB1C3
Source
Submitted by	[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

Nearly all cyan fluorescent proteins are generated on the basis of GFP-like proteins from jellyfish Aequorea macrodactyla. mCyan is an enhanced version of the cyan flourescent protein. The cyan spectral region is from 470 up to 500 nm. mCFP has higher brightness level, acid insensivity and greater photostability. Like GFP, CFP can be used as a reporter gene. So it can be used in vitro for cell culture experiments. In our viral vector system, CFP is used as gene of interest in the vector plasmid. In this content, it is able to detect the efficiency of the constructed viral particles.

Fig.1: Emission spectra and absorbance CFP   http://www.olympusmicro.com/primer/techniques/fluorescence/fret/images/fretintrofigure4.jpg

 

 

Fig.2:Chromophor structure of CFP http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 

Fig.3:  Fluorescent protein beta-barrel architecture and approximate dimensions http://jcs.biologists.org/cgi/reprint/120/24/4247

 

 

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]