Composite

Part:BBa_K322238:Experience

Designed by: William Rostain   Group: iGEM10_Edinburgh   (2010-10-08)
Revision as of 00:58, 28 October 2010 by WillR (Talk | contribs) (→‎Applications of BBa_K322238)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K322238

WTlucie-ph7.jpg

This is a photo of the luciferase when luciferin is added to the LB broth the cells were grown in.

WTlucie-ph4.8.jpg

This is a photo of the luciferase when the cells are resuspended in citrate buffer, pH4.8. This allows better entry of the luciferin into the cells, but also shifts the wavelength of a proportion of the enzymes towards the red end of the spectrum. The yellow colour is due to a mix of green emitting luciferase, and red emitting luciferase.

User Reviews

UNIQ53c9eedd3a240cdf-partinfo-00000000-QINU UNIQ53c9eedd3a240cdf-partinfo-00000001-QINU