Coding
alkB2

Part:BBa_K398001

Designed by: Hugo F. Cueto Rojas and Mathias Voges   Group: iGEM10_TU_Delft   (2010-06-12)
Revision as of 00:21, 28 October 2010 by Mvoges (Talk | contribs)

Medium-chain alkane 1-monooxygenase (Gordonia sp. TF6)

This enzyme belongs to the non-haem di-iron family of monooxygenases and is an integral cytoplasmic membrane protein. It is the catalytic component of the Gordonia sp. TF6 alkane hydroxylase system and as such oxidizes C5-C13 alkanes and C5-C8 cycloalkanes to their corresponding primary alcohols by transferring one oxygen atom from molecular oxygen to the alkane. Together with Rubredoxin 3, Rubredoxin 4 and Rubredoxin Reductase it forms the minimal component alkane hydroxylase system. For more information see the references below.

See BBa_K398017 for the characterized protein generator.

[1] Fujii, T., et al., Biotransformation of various alkanes using the Escherichia coli expressing an alkane hydroxylase system from Gordonia sp TF6. Bioscience Biotechnology and Biochemistry, 2004. 68(10): p. 2171-2177.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
None