Composite

Part:BBa_K319041:Design

Designed by: Afnan Azizi   Group: iGEM10_uOttawa   (2010-10-26)
Revision as of 23:56, 27 October 2010 by Aazizi (Talk | contribs) (→‎Results)

NatMX cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 690
    Illegal NgoMIV site found at 779
    Illegal AgeI site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]


Backgournd

The nat gene was first isolated from Streptomyces noursei[1]. This gene provides the organism with resistance to the antibiotic nourseothricin. In 1999, Goldstein and McCusker produced a nat cassette based on the kanMX cassettes of plasmid pFA6 and showed that this new cassette confers resistance to the above mentioned drug in S. cerevisiae strain YAG44[2]. We have BioBricked the complete cassette (including the upstream TEF1 promoter and the downstream TEF1 terminator) and have shown that it has 100% selectivity in S. cerevisiae YPH500 strain.

Results

Source

n

References