Regulatory

Part:BBa_I752000

Designed by: David Franz   Group: iGEM07_Lethbridge   (2007-08-27)
Revision as of 23:54, 27 October 2010 by Blackrabbit (Talk | contribs)

Riboswitch(theophylline)

The riboswitch changes conformation depending on the presence of theophylline. In the absence of theophylline, the switch prevents the binding of the mRNA to the ribosome whilst in the presence of said chemical, the conformation changes and the mRNA behind the switch can then be expressed.

Part:BBa_K411001 is an improvement of this part. We used the Part:BBa_K411003 to test the construct. [http://2010.igem.org/Team:NYMU-Taipei/Experiments/Speedy_switch#Results Experimental results] from iGEM10's NYMU-Taipei team confirm that the part works and has an increased rate of induction when the concentration of Theophylline increases.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None