Coding
Part:BBa_K322120:Design
Designed by: Meng Lu and William Rostain Group: iGEM10_Edinburgh (2010-10-27)
Green light sensor with LacZ reporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2640
Illegal SpeI site found at 2735 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2640
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2011
Illegal SpeI site found at 2735 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2640
Illegal BamHI site found at 644
Illegal XhoI site found at 2948 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2640
Illegal SpeI site found at 2735 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2640
Illegal SpeI site found at 2735
Illegal NgoMIV site found at 971
Illegal AgeI site found at 2675 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
CcaS and PhoR fusion forms the green light sensor. PhoR and LacZ act as a reporter system. There are still two illegal restriction sites in the sequence.
Source
CcaS: Synechocystis sp. (strain PCC 6803)- Synthesised by Unam Mexico 2010 PhoR: Escherichia coli (strain K12)genomic DNA PhoA promoter: Escherichia coli K12 LacZ reporter: Escherichia coli K12