Composite

Part:BBa_K353002:Design

Designed by: Alejandro Virrueta   Group: iGEM10_Stanford   (2010-10-25)
Revision as of 22:49, 27 October 2010 by Axva1663 (Talk | contribs) (→‎Design Notes)

I0500 + K353010 + B0034 + E0040 + B1006


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1953
    Illegal SapI site found at 961


Design Notes

Karina Padilla, Francisco Cai, Laura O'Brien and Christina Smolke designed the RSID1 tag. It was developed as to not interfere with E. coli's native genome and to be bound by sRNA1 (see part K353009). It also contains a ribosome binding site (B0034).

Source

This part is assembled from other parts found in the registry (except for the RSID tag).

References