Coding

Part:BBa_K322120:Design

Designed by: Meng Lu and William Rostain   Group: iGEM10_Edinburgh   (2010-10-27)
Revision as of 22:17, 27 October 2010 by Registry (Talk | contribs)

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Green light sensor with LacZ reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2640
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2011
    Illegal SpeI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2640
    Illegal BamHI site found at 644
    Illegal XhoI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 2675
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CcaS and PhoR combine to be the sensor biobrick and PhoR and LacZ combine to be the biobrick of reporter part.


Source

CcaS: Synechocystis sp. (strain PCC 6803)- Synthesised by Unam Mexico 2010 PhoR: Escherichia coli (strain K12)genomic DNA PhoA promoter: Escherichia coli K12 LacZ reporter: Escherichia coli K12


References