Regulatory
Part:BBa_K415506
Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk Group: iGEM10_MIT (2010-10-27)
pTRE-Tight L4R1 MammoBlock
This inducible promoter can be switched on by the activity of rttA3 transcription factor. It has been optimized to contain multiple TRE binding sites; this makes the 'tight' version a significant improvement over the previously used simple TRE promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |