Tag

Part:BBa_K496003

Designed by: Shunichi MAKINO   Group: iGEM10_HokkaidoU_Japan   (2010-10-17)
Revision as of 22:07, 27 October 2010 by Laurynas (Talk | contribs)

RBS + SlrP secretion tag, salmonella T3 sec. sys.

leucine-rich repeat protein (SlrP) One of the secretion signals in Salmonella Type 3 Secretion System(T3SS). In theory can be used to secrete tagged proteins through Type 3 Secretion Apparatus(T3SA). Due to functional limitations of T3SA it's known that it can't transport very stable proteins like zinc fingers. Regardless it was shown to transport such stable proteins like GFP.

See [BBa_K496004]

References

  1. Miao EA, Miller SI. 2000. A conserved amino acid sequence directing intracellular type III secretion by Salmonella typhimurium. Proc Natl Acad Sci U S A. Vol.97:7539-7544. [http://www.ncbi.nlm.nih.gov/pubmed/10861017 PubMed]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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