Regulatory
Part:BBa_K354003:Design
Designed by: Gregory Meyer Group: iGEM10_WITS-South_Africa (2010-10-20)
PlcR Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
So as to create a library of promoters with differing transcriptional activities, randomised nucleotides were introduced into the -10 box - the binding site for the RNAP sigma-factor.
Source
The sequence is derived from the PlcR promoter in Bacillus thuringiensis and was synthesized via primer extension.
References
[1] Lereclus D., Agaisse H., Gominet M., Salamitou S. & Sanchis V. (1996). Identification of a Bacillus thuringiensis Gene That Positively Regulates Transcription of the Phosphatidylinostitol-Specific Phospholipase C Gene at the Onset of the Stationary Phase. Journal of bacteriology 178:2749-2756.