Part:BBa_K361001

Message gene template

This message system consists of a unique designated message sequence, being inserted with specific repeat sequence for recognition site by Rci site-specific recombinase.

• This message has 70 characters:

We must learn to live together as brothers or perish together as tools

• In a 4 bits system, the total sequence is 280 bp.

In theory, the sequence with length equal to/ lower than 536 bp has high inversion frequency after 50 generation.

• Reference:

Gyohda, A. & Funayama, N. (1997). Analysis of DNA Inversions in the Shufflon of Plasmid R64. American Society of Microbiology, 179 (6), 1867-1871.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 404
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 7
  Illegal BamHI site found at 427
  Illegal XhoI site found at 433
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]