Measurement

Part:BBa_K313009

Designed by: Ryosuke Kamei, Ryo Karyazono, Kosuke Uekusa   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 21:18, 27 October 2010 by Pokeo (Talk | contribs)

cre recombinase assay device

This is a device with the T7 promoter to detect the function of the Cre recombinase.

If Cre recombinase is expressed in the cell, the double-terminator will be excised irreversibly and GFP will be expressed by the T7 RNA polymerase.

You may be able to quantify the efficiency of the recombination by Cre by measuring the fluorescence intensity of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 940


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Categories
Parameters
None