Generator

Part:BBa_K361011

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM10_Hong_Kong-CUHK   (2010-10-24)
Revision as of 21:08, 27 October 2010 by Jacky Loo (Talk | contribs)

Rci recombinase system

This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.

  • promotor: lac promotor
  • RBS: define efficiency =1
  • Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo>
  • terminator: double bidirectional terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 588
    Illegal NgoMIV site found at 1083
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
//function/recombination
Parameters
biologyactive wide type enzyme
functionSite-specific recombination
protein