Coding

Part:BBa_K317001:Design

Designed by: Yoshiyuki MURAKAMI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)
Revision as of 21:04, 27 October 2010 by Yoshiyuki (Talk | contribs) (→‎Design Notes)

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HtrII (NpHtrII)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 751
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 999
    Illegal BsaI.rc site found at 328
    Illegal BsaI.rc site found at 892
    Illegal SapI site found at 102
    Illegal SapI.rc site found at 964


Design Notes

The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication

Phototaxis Device-1.png Primer sets.png

Source

Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T))

References