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Applications of BBa_K082026
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- Characterization of BBa_K082026
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Tokyo Tech iGEM2010
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Since sequence data of BBa_K082026 is incorrect, please refer to BBa_K395400.
However, we confirmed that both BBa_K395400 and BBa_K082026 have the same activity.
In order to characterize K395400, LacI Mutant, we constructed BBa_K395401 combining I0500 and K082026(K395400) on pSB1A3 and used BBa_I20260 as a positive control and used promoterless gfp on pSB3K3 as a negative control.
Furthermore, we constructed BBa_K395402 combining I0500 and BBa_I 732820 on pSB1A3 as a control experiment.
As a GFP reporter, we used BBa_I7106 on pSB3K3.
In order to measure the function of K395400), we introduced BBa_I7106 (lacI+pL-rbs-GFP-ter) on pSB3K3 and BBa_K395401/BBa_K395402 into DH5α.
We confirmed that product of lacIM1 shows weaker repression to lac promoter than its wild type.
For more information, see [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 our work in Tokyo_Tech 2010 wiki]
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