Reporter

Part:BBa_K407007

Designed by: Syed Habib Tahir Bukhari, Ashwini Rahul Akkineni, Adithya Ananth, Victor Gordeev, Svea Grieb, Sarah Mansour, Mareike Roth, Charanya Sampathkumar, Lucas Schirmer, Jonathan Tam   Group: iGEM10_BIOTEC_Dresden   (2010-10-24)
Revision as of 20:44, 27 October 2010 by Charansam21 (Talk | contribs)

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RFP reporter + LuxI

This part combines a RFP reporter and LuxI and therefore can be used as amplification system for AHL detection. For this purpose a corresponding promoter has to be assembled upstream. LuxI is a synthase that converts SAM into a small molecule calld AHL (acyl-homoserine lactone).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None