Plasmid_Backbone

Part:BBa_K249005

Designed by: Roxanne Shank   Group: iGEM09_Lethbridge   (2009-08-26)
Revision as of 20:32, 27 October 2010 by Liszabruder (Talk | contribs)

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C-terminal Arginine Fusion Vector

pSB1A3 with an C-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the C-terminus of a Protein (Includes Stop Codon)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


This part was designed as a plasmid backbone to allow for easy attachment of a C-terminal Arginine Tag to a gene of choice for localization into the Lumazine Synthase microcompartment.

This part was shown to work vis the sequencing information that we received.

N-terminal tagged YFP did not have substantially more fluorescence than control cells. Cells expressing C-terminal tagged YFP had ten times more fluorescence than control cells and cells expressing N-terminal tagged YFP.

[edit]
Categories
//plasmidbackbone/proteinfusion
//proteindomain/localization
Parameters
tagFuses 10Arg tag to any biobrick silver lab fusion standard (C-terminal)
targetTargets Microcompartments