Coding

Part:BBa_K380009:Design

Designed by: Nina Schiller   Group: iGEM10_Stockholm   (2010-10-26)
Revision as of 19:20, 27 October 2010 by JohanNordholm (Talk | contribs) (→‎Design Notes)

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Protein A Z-domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 44
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start and stop codon was removed and the gene was redesigned with BioBrick prefix and suffix according to assembly standard 25 using specifically designed primers and PCR.

Source

Organism: Staphylococcus aureus subsp. aureus MRSA252 (strain: MRSA252, sub-species: aureus MRSA252) http://www.ncbi.nlm.nih.gov/gene/2859152

References