Coding

Part:BBa_K317008

Designed by: Yuta Sakai   Group: iGEM10_Tokyo-NoKoGen   (2010-10-19)
Revision as of 18:02, 27 October 2010 by Yuta (Talk | contribs)

Antigen 43

Antigen 43 involves in auto-aggregation of the cell. By expressing Antigen 43, the cell will aggregate when the culture solution is stood. Antigen 43 is derived from E. coli genome. The wildtype of Antigen 43 contains 6 PstI sites but this part does not contain any of them. So, this parts can be used as a normal BioBrick which is able to ligate 2 parts by 3A assembly.

The function of this parts is evaluated by BBa_K371024 and BBa_K317026.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
    Illegal NgoMIV site found at 1933
    Illegal NgoMIV site found at 2443
    Illegal NgoMIV site found at 2464
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2203
    Illegal AgeI site found at 2617
    Illegal AgeI site found at 2859
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAntigen 43