Coding

Part:BBa_K380006:Design

Designed by: Johan Nordholm   Group: iGEM10_Stockholm   (2010-10-26)
Revision as of 17:35, 27 October 2010 by JohanNordholm (Talk | contribs) (→‎Source)

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Human basic fibroblast growth factor, bFGF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 403
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


Design Notes

An AgeI restriction site was situated at nt 340-345. As assembly standard 25 was used the restriction site was removed with site-directed mutagenesis, by changing nt 341 from C to T.


Source

Human bFGF-GST in pGEX was kindly provided by Nozomu Nishi, Kagawa University, Japan [1].

[1] Collagen-binding growth factors: production and characterization of functional fusion proteins having a collagen-binding domain, 1998

References