Composite

Part:BBa_K389014

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-09-28)
Revision as of 17:03, 27 October 2010 by TimoW (Talk | contribs)

VirA/G screening system test

This BioBrick is made to test our virA screening system and the strategy for directed evolution, respectively. It contains an unmutated virA under the control of a constitutive promoter and the screening device BBa_K389011.


Usage and Biology

This Device can be used for characterization and screening of mutated VirA receptors.

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389015


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768


[edit]
Categories
//chassis/prokaryote/ecoli
//test
Parameters
None