Part:BBa_K398206
AlnA generator (IPTG inducible)
AlnA is a protein with emulsification activity originated from the natural oil degrading bacterium Acinetobacter radioresistens. It has been shown that this protein keeps its emulsifying capacitiesis when it is produced by other organisms, such as Escherichia coli.
AlnA has an amphipathic structure, which allows it to form micelles that accumulate at the interface between liquids of different polarities such as water and oil. This process is based upon the ability of biosurfactants to reduce surface tension, blocking the formation of hydrogen bridges and certain hydrophilic and hydrophobic interactions. The emulsifier helps to disperse the oil by emulsifying the oil, thus increasing the surface area for the growth of microorganisms on hydrocarbons.
The original sequence is available in GenBank http://www.ncbi.nlm.nih.gov/nuccore/17978529. We optimized it for expression in E. coli and removed unwanted restriction sites.
The expression of AlnA, induced by IPTG, has been shown to increase the solubility of the hydrophobic dye Sudan II by 20% in cell extract assays. The full [http://2010.igem.org/Team:TU_Delft#page=Project/solubility characterization of the BBa_K398206] is described on the TU Delft iGEM Team 2010 wiki.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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