Composite
406

Part:BBa_K398406:Design

Designed by: Hugo Federico Cueto Rojas and Ramon van der Valk   Group: iGEM10_TU_Delft   (2010-10-12)
Revision as of 16:38, 27 October 2010 by Hugo 87 (Talk | contribs) (→‎Source)

Solvent tolerance cluster


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter.


Source

Genomic sequence obtained from NCBI.

Prefoldin alpha accession number: [http://www.ncbi.nlm.nih.gov/gene/1444414 PH0527]

Prefoldin beta accession number: [http://www.ncbi.nlm.nih.gov/gene/1444421 PH0532]

References