Generator
Ori+Kan

Part:BBa_K320004:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 16:04, 27 October 2010 by Perrudet (Talk | contribs) (→‎Design Notes)

Origin for Asaia and resistance to Kanamycin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal AgeI site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4


Design Notes

We ligate BioBricks BBa_K320000 and BBa_P1003

The first BioBrick (BBa_K320000) was intial in the pSB1C3 backbone vector. We opened it with the restriction enzyme SpeI and PstI.

The second BioBrick (BBa_P1003) was also stored in the pSB1C3 backbone vector. This one we digested with Xba and PstI.

Source

The BioBricks assembled from BBa_K320000 and BBa_P1003.

References