Coding
TdcE

Part:BBa_K348000:Design

Designed by: Laurence Van Melderen   Group: iGEM10_ULB-Brussels   (2010-10-27)
Revision as of 15:28, 27 October 2010 by ULB (Talk | contribs) (→‎Design Notes)

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TdCE - pyruvate formate lyase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1107
    Illegal AgeI site found at 511
    Illegal AgeI site found at 1861
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was amplified by PCR using those primers :

CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT

CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT

No special safety issues exist surrounding this part.

Source

It comes from the genome of Escherichia coli K-12 substr. MG1655

References

[http://ecoliwiki.net/colipedia/index.php/ECK3103] [http://biocyc.org/ecoli/new-image?object=G7627]