Part:BBa_K398406
Solvent tolerance cluster BBa_K398406 enables E.coli of working in two-phase systems with resistance to high concentrations of hydrophobic molecules in the membrane. A growth rate increase of 62% in comparison to a standard E.coli was achieved at concentrations of 8% (v/v) n-hexane.
Introduction
The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from Pyrococcus horikoshii OT3. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes. [[(img src="https://static.igem.org/mediawiki/2010/2/2f/TU_Delft_SaltModel.jpg">|500px|center|thumb|figure 1: Model of the Solvent tolerance]]
The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
- 1000COMPATIBLE WITH RFC[1000]
| biology | 63% growthrate increase at 8% (v/v) n-hexane |