Coding
TdcE

Part:BBa_K348000:Design

Designed by: Laurence Van Melderen   Group: iGEM10_ULB-Brussels   (2010-10-27)
Revision as of 15:17, 27 October 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


TdCE - pyruvate formate lyase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1107
    Illegal AgeI site found at 511
    Illegal AgeI site found at 1861
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was amplified by PCR using those primers : CCCGAATTCGCGGCCGCTTCTAGATGAAGGTAGATATT

CCCCTGCAGCGGCCGCTACTAGTATCAGAGCGCCTGGGT


Source

It comes from the genome of Escherichia coli K-12 substr. MG1655

References