Coding

Part:BBa_I15010:Design

Designed by: Jeff Tabor   Group: iGEM04_UTAustin   (2004-09-20)
Revision as of 01:22, 5 June 2006 by Registry (Talk | contribs)


cph8 (Cph1/EnvZ fusion)


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Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2257
    Illegal XhoI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutation at base 108 (G-A) to remove PstI site


Source

Voigt Lab via Anselm Levskaya

References

  • Gambetta and Lagarias. Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10566-71.