Regulatory
luxR

Part:BBa_R0061:Experience

Designed by: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu   Group: Antiquity   (2004-01-28)
Revision as of 14:20, 27 October 2010 by Onoda (Talk | contribs) (→‎iGEM Chiba 2010)

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Applications of BBa_R0061

User Reviews

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No review score entered. iGEM Tokyo_Tech 2010
In order to characterize R0061, Plux repression promoter, we constructed K395101 combining R0061 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1 and used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control.

Overnight cultures of reporter strains grown at 37 °C containing appropriated antibiotics were diluted at least 1:100 and incubated at 37 °C as fresh cultures. After their OD590 reached 0.6, added 3OC6HSL. After 3 hours of induction, fluorescence intensity was measured with flow cytometry.

After 3 hours of induction by 3OC6HSL, the expression of GFP with 3OC6HSL dropped to 1/3 comparing with the expression without 3OC6HSL.

We confirmed that this promoter works correctly.


Tokyotech R0061 K395008 graph R0061.jpg

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iGEM Chiba 2010

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