Regulatory
luxR

Part:BBa_R0061:Experience

Designed by: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu   Group: Antiquity   (2004-01-28)
Revision as of 14:20, 27 October 2010 by Onoda (Talk | contribs) (→‎iGEM Chiba 2010)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_R0061

User Reviews

UNIQ50f693ffcceb5e63-partinfo-00000000-QINU

No review score entered. iGEM Tokyo_Tech 2010
In order to characterize R0061, Plux repression promoter, we constructed K395101 combining R0061 and K121013, which is a promoter-less gfp reporter (rbs-gfp-ter-ter) on pSB6A1 and used a fusion of PlacIq (I14032) to gfp (K121013) as a positive control and used promoterless gfp (K121013) as a negative control.

Overnight cultures of reporter strains grown at 37 °C containing appropriated antibiotics were diluted at least 1:100 and incubated at 37 °C as fresh cultures. After their OD590 reached 0.6, added 3OC6HSL. After 3 hours of induction, fluorescence intensity was measured with flow cytometry.

After 3 hours of induction by 3OC6HSL, the expression of GFP with 3OC6HSL dropped to 1/3 comparing with the expression without 3OC6HSL.

We confirmed that this promoter works correctly.


Tokyotech R0061 K395008 graph R0061.jpg

UNIQ50f693ffcceb5e63-partinfo-00000002-QINU


iGEM Chiba 2010

we also cheracterized