Generator
S + P25 +

Part:BBa_K320014:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-24)
Revision as of 13:11, 27 October 2010 by Spaceman (Talk | contribs) (→‎Source)

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S + P25 + P28 + Ori + Tet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal NheI site found at 827
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2699
    Illegal AgeI site found at 2859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1537


Design Notes

The P protein sequences were very AT-rich and not optimized for Asaia and E.Coli. No expression took place.

Source

This part has been designed from the following parts: BBa_K320003, BBa_K320007 and BBa_K320009.

References