Composite

Part:BBa_K395401:Experience

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 12:59, 27 October 2010 by ODDY (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K395401

In order to measure the BBa_K082026/BBa_K395400, we designed this part.
We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.



Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera



User Reviews

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