Device

Part:BBa_K427007

Designed by: Diana Ostos Rangel   Group: iGEM10_Tec-Monterrey   (2010-10-22)
Revision as of 10:41, 27 October 2010 by Janmarte (Talk | contribs)

Ter PBadweak Mu C Ter Pmom GFP cassette

This part was used for the characterization of the Mu C sensitivity tuner. It is composed of seven biobricks starting with Ter PBadweak, which is the part created by British Columbia team in 2007 starts with a terminator and a promoter that respond to high concentrations of Arabinose. It is followed by sequence coding for C protein which inter activates the Pmom promoter and this enhance the PoPS activity. Then is followed by the GFPcassette sequence a biobrick.

This part has no special safety considerations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 168
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1542

References

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Categories
Parameters
None