![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
PompC(C)
Part:BBa_K395301
Designed by: THIPRAMPAI THAMAMONGOOD Group: iGEM10_Tokyo_Tech (2010-10-02)
PompC(C) Promoter regulated by phosphorylated OmpR
This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(C) promoter consists of binding site 1 and additional 4 base pairs before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]
![](/wiki/images/6/6d/Tokyotech_ompc_graph.jpg)
Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. We characterized this promoter by attaching GFP(E0240) to the downstream and mearsuring its transcriptional activity through the GFP expression.
This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010
This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA Tokyo Tech iGEM2010
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 24
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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