Device

Part:BBa_K317039

Designed by: Yasuhiko SASAKI   Group: iGEM10_Tokyo-NoKoGen   (2010-10-21)
Revision as of 08:15, 27 October 2010 by Yasuhiko (Talk | contribs)

Pconst.(H)-RBS-LuxR-Double term-Plux-Lysis

This device senses the AHL concentration in the media and regulates the expression of downstream genes. The complex of the LuxR protein and the AHL activates the promoter, increasing the rate of transcription. Also, This devicce includes BBa_K112808 at the downstream gene, the auto lysis device which causing lysis of E.coli



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2400
    Illegal NheI site found at 2423
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2005
    Illegal AgeI site found at 2075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal SapI site found at 2656


[edit]
Categories
Parameters
None