Measurement

Part:BBa_K313018

Designed by: Ryosuke Kamei, Kosuke Uekusa, Ryo Kariyazono   Group: iGEM10_UT-Tokyo   (2010-10-22)
Revision as of 07:40, 27 October 2010 by Pokeo (Talk | contribs)

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B1006. If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part. With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, we can detect the quantity of Cre and can assess the leakiness of the terminator. The difference between this part and BBa K313017 is the number of the terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None