Part:BBa_K391007

Constitutive promoter-RBS-DspB

Strongest constitutive promoter in J23100 family (J23100) + strongest RBS in the community collection (B0034)+ Dispersin B carbohydrate digesting enzyme (K391006).

Characterization Data

The activity of DspB was tested by taking a crude cell lysate from E.coli cells expressing the protein. The protein in the lysate was then tested against a chromogenic substrate: 4-Nitrophenyl N-acetyl-β-D-glucosaminide. As DspB cleaves this substrate, the absorbance shifts from 300nm (substrate: 4-Nitrophenyl N-acetyl-β-D-glucosaminide) to 405nm (product: 4-nitrophenol). We measured the change in the level of absorbance at 405nm over time and the result is shown below.

The graph above plots the absorbance at wavelength 405nm (y-axis) over time in days (x-axis). It can be observed from the graph that over time, the absorbance at 405nm increases with the treatment with the lysate containing DspB by a significant amount compared to the controls. This assay has been replicated and the results have shown to be reproducible.