Generator

Part:BBa_K395146

Designed by: Misaki Kaneko   Group: iGEM10_Tokyo_Tech   (2010-10-20)
Revision as of 07:03, 27 October 2010 by Misaki (Talk | contribs)

constitutive LuxI generator

This is a constitutive LuxI generator using P(Lac)IQ (High Transcription) with RBS and Terminator.

The level of activity of K395146 is equal to about 10 nM 3OC6HSL.


Tokyotech Fig.K395146-1.jpg
Tokyotech Fig.K395146-1.jpg

We measured 3OC6HSL production by addition of supernatant from liquid culture of K395146 into a reporter cell which expresses GFP in presence of LuxR/3OC6HSL. Fig1 shows GFP fluorescence increased in presence of the supernatant of liquid culture with LuxI-expression cell, which proved 3OC6HSL was synthesized by K395146. Fig2 shows the relationship between GFP fluorescence and the concentration of 3OC6HSL. Taking account of this graph, the level of activity of K395146 is equal to about 10 nM 3OC6HS.











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None