Regulatory
PompC-CS1

Part:BBa_K395303

Designed by: Taichi Nakamura   Group: iGEM10_Tokyo_Tech   (2010-10-02)
Revision as of 05:41, 27 October 2010 by Taichi (Talk | contribs)

PompC(CS1) Promoter regulated by phosphorylated OmpR

This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(CS1) promoter consists of only binding site 1 before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series] |width='60%' valign='top'|

Figure 1. Induction of new OmpC series in high osmolarity medium Tokyo Tech iGEM2010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None