Regulatory

Part:BBa_K337057

Designed by: Rebecca Berrens   Group: iGEM10_Heidelberg   (2010-10-26)
Revision as of 04:58, 27 October 2010 by Registry (Talk | contribs)

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synthetic binding site of miR 122 (imperfect) KD: 24%

imperfect binding site of miR122 in the tuning construct leads to a knockdown percentage of 64% percent if introduced into the 3'UTR of the gene of interest (GOI). part can be either co-transfected with a synthetic miRNA or transfected into liver cell lines (i.e. Huh7).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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