Coding
vioB-AB

Part:BBa_K349110

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 04:39, 27 October 2010 by AlinaP (Talk | contribs)

vioB-AB (BioBytes 2.0)

A component of the Vio Operon which is used to produce a light green pigment from L-tyrosine. Depending on which parts of the operon are available, a different colour will be produced. Please note this piece is "naked", meaning it does not contain a promoter, an RBS, or start and stop codons. The promoter and terminator is intended to be reconstituted through the adjoining BA BioBytes attached through the BioBytes 2.0 assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2529
    Illegal AgeI site found at 2725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 3006


[edit]
Categories
Parameters
n/avioB-AB (BioBytes 2.0)