Coding
ampR-BA

Part:BBa_K349302:Design

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 04:03, 27 October 2010 by AlinaP (Talk | contribs) (→‎Design Notes)

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ampR-BA (BioBytes 2.0)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 960


Design Notes

This part has built-in BsaI recognition sites surrounding a P1002 ampicillin resistance cassette. Digesting at the BsaI cut sites inside of the prefix and suffix will create a B overhang at the 5' end and an A overhang at the 3' end. These ends are necessary for use in the BioBytes 2.0 assembly method.

Source

pSB1C3

References